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11.
Alex Buchberger Harpinder Saini Kiarash Rahmani Eliato Azadeh Zare Ryan Merkley Yang Xu Julio Bernal Robert Ros Mehdi Nikkhah Dr. Nicholas Stephanopoulos 《Chembiochem : a European journal of chemical biology》2021,22(10):1755-1760
Biomaterials with dynamically tunable properties are critical for a range of applications in regenerative medicine and basic biology. In this work, we show the reversible control of gelatin methacrylate (GelMA) hydrogel stiffness through the use of DNA crosslinkers. We replaced some of the inter-GelMA crosslinks with double-stranded DNA, allowing for their removal through toehold-mediated strand displacement. The crosslinks could be restored by adding fresh dsDNA with complementary handles to those on the hydrogel. The elastic modulus (G’) of the hydrogels could be tuned between 500 and 1000 Pa, reversibly, over two cycles without degradation of performance. By functionalizing the gels with a second DNA strand, it was possible to control the crosslink density and a model ligand in an orthogonal fashion with two different displacement strands. Our results demonstrate the potential for DNA to reversibly control both stiffness and ligand presentation in a protein-based hydrogel, and will be useful for teasing apart the spatiotemporal behavior of encapsulated cells. 相似文献
12.
为了实现在工业化生产中对α钛富氧层厚度预测和控制,通过实验研究α钛富氧层在高温空气环境中的形成及增厚过程,讨论热处理温度和时间的影响作用,建立高温(750~850℃)空气环境下关于温度、时间的富氧层增厚动力学模型。结果表明:当恒温热处理温度为750~850℃时,α钛富氧层厚度x与保温时间t0.5呈正比例关系,且升高热处理温度可显著提高富氧层增厚速度。在此温度范围内,氧原子的扩散激活能约为203473 J/mol,计算曲线与实验数据吻合性较好。结合文献中已有的扩散系数方程和实验测得的富氧层厚度数据,推导得到5个富氧层增厚动力学方程,其中3个方程的计算曲线与实验数据吻合性较好,可为实际生产中预估富氧层厚度提供理论支持。 相似文献
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梯度分层铝合金蜂窝板是一种有效的吸能结构,本工作在梯度铝蜂窝结构的基础上根据梯度率的概念,通过改变蜂窝芯层的胞壁长度,设计了4种质量相同、梯度率不同的铝蜂窝夹芯结构。通过准静态压缩实验,并结合非线性有限元模拟准静态及冲击态下梯度铝蜂窝夹芯结构的变形情况及其力学性能,分析对比了相同质量下梯度铝蜂窝夹芯结构在准静态下的变形模式以及冲击载荷下分层均质蜂窝结构和不同梯度率的分层梯度蜂窝结构的动态响应和能量吸收特性。结果表明:在准静态压缩过程中,铝蜂窝梯度夹芯板的变形具有明显的局部化特征,蜂窝芯的变形为低密度优先变形直至密实,层级之间的密实化应变差随芯层密度的增大而逐渐减小;在高速冲击下,梯度蜂窝板并非严格按照准静态过程中逐级变形直至密实,而是在锤头冲击惯性及芯层密度的相互作用下整体发生的线弹性变形、弹性屈曲、塑性坍塌及密实化;另外,在本工作所设计的梯度率中,当梯度率为γ1=0.0276时,梯度蜂窝夹芯板的吸能性达到最好,相较于同等质量下的均质蜂窝夹芯板,能量吸收提高了10.63%。 相似文献
15.
Yingfeng XU Youiun HU Xiaodong ZHANG Xingyuan XU Lei YE Xiaotao XIAO Zhen ZHENG 《等离子体科学和技术》2021,23(9):95102-23
NBI fast ion losses in the presence of the toroidal field ripple on EAST have been investigated by using the orbit code GYCAVA and the NBI code TGCO. The ripple effect was included in the upgraded version of the GYCAVA code. It is found that loss regions of NBI fast ions are mainly on the low field side near the edge in the presence of ripple. For co-current NBIs, the synergy effect of ripple and Coulomb collision on fast ion losses is dominant, and fast trapped ions located on the low field side are easily lost. The ripple well loss and the ripple stochastic loss of fast ions have been identified from the heat loads of co-current NBI fast ions. The ripple stochastic loss and the collisioninduced loss are much larger than the ripple well loss. Heat loads of lost fast ions are mainly localized on the right side of the radio frequency wave antennas from the inside view toward the first wall. For counter-current NBIs, the first orbit loss due to the magnetic drift is the dominant loss channel. In addition, fast ion loss fraction with ripple and collision for each NBI linearly increases with the effective charge number, which is related to the pitch angle scattering effect. 相似文献
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17.
《Ceramics International》2022,48(6):7864-7875
Based on the ultrasonic C-scan results of 8YSZ coatings after thermal cycles, three-dimensional cylindrical numerical simulations of the physical geometry model of the thermal barrier coating (TBC) sinusoidal surfaces were conducted with finite elements to estimate the stress distribution and evolution law of the top coat (TC)/bond coat (BC) interface, including the centre and edge of the specimen affected by the dynamic growth of the thermally grown oxide (TGO). The results show that when a layer of TGO is grown on the TC/BC interface, compressive stress is uniformly distributed on the TGO interface, and the stress value decreases as a function of the TGO layer thickness. When the thickness of the TGO exceeds a certain value, the compressive stress of all parts of the interface gradually changes to tensile stress; meanwhile, the edges of the model affected by the crest and trough effects of the wave are reflected in the radial and circumferential directions, especially along the axial direction, with alternating concentrated tensile and compressive stresses. TGO growth imposes a minor influence on the magnitude and distributions of the radial and circumferential stresses at the BC interface. The linear elasticity, creep, fatigue, and stress accumulation effects of each layer of TBCs in each thermal cycle were fully considered in this model. The model not only interprets the crest and trough effects of the TC/BC surface interface during the growth of TGO, but also interprets the effects of the core and edge of the cylindrical model, further revealing the reason for which the core and edge of the TBC will most likely form cracks. 相似文献
18.
Rong Yang Anqi Xu Yanting Chen Nan Sun Jinjie Zhang Ru Jia Tao Huang Wenge Yang 《Journal of texture studies》2020,51(6):968-978
In order to ameliorate the gel quality of Dosidicus gigas surimi, the effects of laver powder on gel properties, rheological properties, and water-holding capacity (WHC) were investigated. Results indicated that the addition of laver powder could significantly increase the hardness, chewiness, and breaking force of surimi gels. However, the texture indexes and gel strength began to decline when additional amount exceeded 0.6%. Rheological results demonstrated that the addition of laver powder increased the storage modulus (G′) and viscosity of surimi, prolonged protein denaturation temperature in surimi gels. Moreover, the WHC of surimi gel was improved with the increase of laver powder. Further analyses in low-field nuclear magnetic resonance revealed that laver powder could shorten the transverse relaxation time, enhanced the combination with water, and altered the distribution of different water categories. The proportion of bound water and immobilized water reached its maximum and minimum at 0.6% of laver powder, respectively. Correlation analyses showed that WHC of surimi gel was negatively correlated well with the proportion of loose-bound water, but positively correlated with the strong-bound water and free water. In conclusion, the results supported that 0.6% was the optimal additional amount of laver powder for the squid-based surimi production based on the current ingredients of surimi products. 相似文献
19.
在五轴动力总成试验台架上对牵引车在不同载荷下进行同一路谱的排放试验,研究牵引车在不同载荷下NOx排放和排温的变化,得到载荷与排放物和选择性催化还原(selective catalytic reduction,SCR)上游排温的变化关系。结果表明:载荷为标定载荷的60%以下时,NOx比排放值与载荷基本呈线性相关;载荷超过标定载荷的75%时,NOx比排放值与载荷呈二次项相关;载荷在为标定载荷的23%以上时,NOx比排放值与SCR上游排温基本呈线性相关。利用该试验结论可以通过只进行单一载荷排放试验,判断车辆在其他载荷下的排放水平是否满足国家标准要求,并可通过载荷对排温的影响规律预判排放水平。 相似文献
20.
随着基于位置社交网络(Location-Based Social Network,LBSN)的快速发展,兴趣点(Point-Of-Interest,POI)推荐可以帮助人们发现有趣的并吸引人的位置。针对签到数据的稀疏性和用户兴趣的动态性等挑战性问题,提出了基于LBSN动态异构网络的时间感知兴趣点推荐算法。在LBSN异构网络模式中增加会话节点类型。通过动态元路径,在用户和兴趣点语义关系之间有效地融入时间信息、位置信息和社交信息等。设置了用户-兴趣点之间的动态元路径集,并提出了动态路径实例的偏好度计算方法。采用矩阵分解模型对不同动态偏好矩阵进行矩阵分解。根据不同动态元路径的用户特征矩阵和兴趣点特征矩阵,获取用户在目标时间访问兴趣点的推荐列表。实验结果表明,与其他兴趣点推荐方法相比,所提方法在兴趣点推荐精确度上取得了较好的推荐结果,具有良好的应用前景。 相似文献